Generator

Part:BBa_K4628027:Design

Designed by: Qinyao Yan   Group: iGEM23_ZJU-China   (2023-10-11)


Pikm-1 expression circuit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1721
    Illegal SpeI site found at 2295
    Illegal SpeI site found at 2301
    Illegal PstI site found at 2514
    Illegal PstI site found at 2730
    Illegal PstI site found at 2745
    Illegal PstI site found at 2814
    Illegal PstI site found at 3258
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3844
    Illegal SpeI site found at 2295
    Illegal SpeI site found at 2301
    Illegal PstI site found at 2514
    Illegal PstI site found at 2730
    Illegal PstI site found at 2745
    Illegal PstI site found at 2814
    Illegal PstI site found at 3258
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2950
    Illegal BamHI site found at 2575
    Illegal XhoI site found at 3408
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1721
    Illegal SpeI site found at 2295
    Illegal SpeI site found at 2301
    Illegal PstI site found at 2514
    Illegal PstI site found at 2730
    Illegal PstI site found at 2745
    Illegal PstI site found at 2814
    Illegal PstI site found at 3258
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1721
    Illegal SpeI site found at 2295
    Illegal SpeI site found at 2301
    Illegal PstI site found at 2514
    Illegal PstI site found at 2730
    Illegal PstI site found at 2745
    Illegal PstI site found at 2814
    Illegal PstI site found at 3258
    Illegal NgoMIV site found at 2329
    Illegal NgoMIV site found at 3426
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1142
    Illegal BsaI site found at 2559
    Illegal BsaI.rc site found at 1096
    Illegal SapI site found at 1497
    Illegal SapI.rc site found at 885
    Illegal SapI.rc site found at 3467


Design Notes

None


Source

Combined with CaMV 35S promoter, Pikm-1, CaMV poly(A) signal

References